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Part:BBa_K598024:Design

Designed by: Yan GONG, Xiaowei YAN   Group: iGEM11_pku_riboclamp   (2011-09-28)


Bistable Switch with TPP Down-regulated Hammerhead Ribozyme 2.5 Regulating CI434 Expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1665
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 10
    Illegal AgeI site found at 122
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2826


Design Notes

We utilize down-regulating TPP ribozyme TPP-2.5 affecting the green-red ratio of bistable switch in order to test our model.


Source

We construct this part by Gibson Assembly with the bistable switch part from Peking 2007 iGEM team and the part BBa_K598003

References